References

The following two papers describe the basic algorithm used to create a 3D object from a stack of images - each image taken at a different focal length. The two papers cover the same algorithm and are quite similar.
- Shape from Focus: Fully Automated 3D Reconstruction and Visualization of Microscopic Objects,by Marcus Niederost, Jana Niederost, and Jiri Scucka.
- Automatic 3D Reconstruction and Visualization of Microscopic Objects From Monoscopic Multifocus Image Sequence, by Marcus Niederost, Jana Niederost, and Jiri Scucka.